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KMID : 1025520040460040625
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Journal of Animal Science and Technology
2004 Volume.46 No. 4 p.625 ~ p.634
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Characteristics of Lactate Dehydrogenase Produced from Lactobacillus sp. FFy111-1 as a Ruminant Probiotic
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Sung H. G.
Kim Dong-Kyun
Bae H. D.
Shin H. T.
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Abstract
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The objective of this experiment is to study the possibility of lactate dehydrogenase(LDH) enzyme to prevent lactate accumulation in the rumen. For understanding capacity of bacterial LDH in rumen environments, this study was conducted to explore the effects of temperature, pH, VFAs and metal ions on Lactobacillus sp. FFy111-1’s LDH activity, and the LDH activation in rumen fluid accumulated lactate. The optimum pH and temperature of LDH were pH 7.5 and 40℃, respectively. The LDH activity had a good thermostability at range from 30 to 50℃. The highest pH stability of the enzyme was at ranges from pH 7.0 to 8.0 and the enzyme activities showed above 64% level of non-treated one at pH 6.0 and 6.5. The LDH was inactivated by VFAs treatments but was enhanced by metal ion treatments without NaCl and CuSO4. Especially, the LDH activity was increased to 127% and 124% of its original activity by 2 mM of BaCl2 and MnSO4 addition, respectively. When the acidic rumen fluid was treated by LDH enzyme of Lactobacillus sp. FFy111-1, the lactate concentration in the rumen fluid was lower compared with non-treated rumen fluid(P<0.05). This lactate reduction was resulted from an action of LDH. It was proved by result of purified D,L-LDH addition that showed the lowest lactate concentration among the treatments(P<0.05). Although further investigation of microbial LDH and ruminal lactate is needed, these findings suggest that the bacterial LDH has the potential capability to decrease the lactate accumulated in an acidic rumen fluid. Also, screening of super LDH producing bacteria and technical development for improving enzyme activity in rumen environment are essential keys for practical application.
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KEYWORD
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Lactate dehydrogenase, Lactobacillus sp., Lactate accumulation
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